Abstract The objective of this study was to determine a nutrients (calcium, magnesium, iron, copper, manganese, zinc and potassium) from manure in around Loei province. The manure samples (cow dung, pig dung and buffalo dung) were collected from 14 sample points. The analysis showed that, cow dung from Muang District of Loei province showed the highest of Calcium content of 25.1070 mg/kg. Pig dung from Nonghin District showed the highest of magnesium content of 42.6460 mg/kg. Pig dung from Muang District showed the highest of iron content of 29.0060 mg/kg. Pig dung from Nonghin District showed the highest of copper content of 2.3502 mg/kg. The highest of manganese content found in pig dung from Nonghin District was 29.1599 mg/kg. The highest of zinc content found in pig dung from Nonghin District was 35.2195 mg/kg. The highest of potassium content found in pig dung from Phakhao District was 14.6863 mg/kg. Percent recovery in this study between 80-110 %, it this acceptable. The LOD value and LOQ value of calcium, magnesium, iron, copper, manganese, zinc and potassium was equal to 0.8157- 2.7176, 3.5614 - 11.7927, 0.8332- 2.5902, 0.1221- 0.1907, 0.0487- 0.1572, 0.0618 - 0.1871 and 0.2736 - 0.8854 mg/kg, respectively.