บทความ (Articles)http://dspace.bru.ac.th/xmlui/handle/123456789/532024-03-29T00:20:24Z2024-03-29T00:20:24Z-Lomarak, Nuansai, Bancha, Promden, Worrawat and Sangsila, Arunrassame Teppornhttp://dspace.bru.ac.th/xmlui/handle/123456789/60042020-03-22T08:20:44Z0031-10-19T00:00:00Z-
Lomarak, Nuansai, Bancha, Promden, Worrawat and Sangsila, Arunrassame Tepporn
The purpose of the study was to investigate the effects of a professional development instructional program Integrated STEM for elementary schools in a rural context of Thailand which carried out at the Faculty of Education, Buriram Rajabhat University during February 2018. Research instruments were (1) assessment observational record consisted of 5 elements of competencies with 4 levels of rubric criteria including beginning, approaching proficient, proficient, and distinguished levels, (2) multiple choice and short answer response achievement test items designed to assess specific science content knowledge. Five essential elements of teaching competencies and rubric criteria with four levels were synthesized and validated by group of 3 experts according to focus group meeting and interview. The research findings revealed that the integrated STEM program was highly recommended to use for developing in-service science teachers’ teaching competencies. From evaluation of experts, the results indicated that competencies’ levels of teaching of science teachers was achieved as approaching proficient level (Mean = 2.48, S.D.=0.41)) in 5 aspects of assessment including --planning for STEM instruction, classroom environment and STEM classroom learning management, strategies for encouraging student to learn effectively, feedback and assessment, and teaching reflection—with approaching proficient level of assessment. Moreover, the results of one sample t-test indicated that teachers' achievement score on science content knowledge after implementing the integrated STEM program was significantly higher than before at the 0.01 level. It can be suggested that in-service training programs should be developed and used for science teachers to raise their awareness of the necessity of STEM education and to enhance their competencies in teaching preparation program.
0031-10-19T00:00:00ZAntioxidant and antityrosinase activities in germinated brown rice of indigenous Thai cultivarsSangsila, arunrussameeWitaya, PimdaWorrawat, Promdenhttp://dspace.bru.ac.th/xmlui/handle/123456789/55772019-09-10T10:37:57Z2018-01-01T00:00:00ZAntioxidant and antityrosinase activities in germinated brown rice of indigenous Thai cultivars
Sangsila, arunrussamee; Witaya, Pimda; Worrawat, Promden
The antioxidant and antityrosinase activities were examined in germinated brown rice
of three indigenous Thai cultivars, namely Riceberry (purple), KDML 105 R-PSL-2 (red) and
KDML 105 (white), which are commonly grown in Buriram province of Thailand. Germination
was induced by steeping brown rice of each cultivar in distilled water (water: grain ratio = 2:1)
at ambient temperature for 6 h. After low temperature induction at 8 – 10 ºC for 24 h, the rice
kernels were allowed to germinate in a double-layered cotton cloth in the dark at ambient
temperature for 0, 24 and 48 h, in which the antioxidant and antityrosinase activities of
germinated brown rice were evaluated. The results revealed that germinated brown rice of all
studied cultivars appeared to display higher levels of antioxidant and antityrosinase activities
than ungerminated brown rice. The results showed 24-48 h germination regimes were most
effective in maximizing the antioxidant and antityrosinase activities in germinated brown rice.
Under such conditions, the maximum quantities of total phenolics, total flavonoids and
antityrosinase were detected in Riceberry (25.06 mg GAE/g DW), KDML 105 R-PSL-2 (95.67
mg QE/g DW) and Riceberry (31.33%), respectively. Again, the highest DPPH value of
81.52% was detected in Riceberry and the highest ABTS value of 67.92% in KDML 105 RPSL-
2. Additionally, a strong correlation between antioxidant, antityrosinase and germination
time was detected. The information obtained from this study should provide a foundation for
future research aiming at the development of functional foods and/or cosmetics.
2018-01-01T00:00:00ZAntioxidant and antityrosinase activities in germinated brown rice of indigenous Thai cultivarsSangsila, arunrussameehttp://dspace.bru.ac.th/xmlui/handle/123456789/55762019-09-10T10:29:58Z2018-01-01T00:00:00ZAntioxidant and antityrosinase activities in germinated brown rice of indigenous Thai cultivars
Sangsila, arunrussamee
The antioxidant and antityrosinase activities were examined in germinated brown rice
of three indigenous Thai cultivars, namely Riceberry (purple), KDML 105 R-PSL-2 (red) and
KDML 105 (white), which are commonly grown in Buriram province of Thailand. Germination
was induced by steeping brown rice of each cultivar in distilled water (water: grain ratio = 2:1)
at ambient temperature for 6 h. After low temperature induction at 8 – 10 ºC for 24 h, the rice
kernels were allowed to germinate in a double-layered cotton cloth in the dark at ambient
temperature for 0, 24 and 48 h, in which the antioxidant and antityrosinase activities of
germinated brown rice were evaluated. The results revealed that germinated brown rice of all
studied cultivars appeared to display higher levels of antioxidant and antityrosinase activities
than ungerminated brown rice. The results showed 24-48 h germination regimes were most
effective in maximizing the antioxidant and antityrosinase activities in germinated brown rice.
Under such conditions, the maximum quantities of total phenolics, total flavonoids and
antityrosinase were detected in Riceberry (25.06 mg GAE/g DW), KDML 105 R-PSL-2 (95.67
mg QE/g DW) and Riceberry (31.33%), respectively. Again, the highest DPPH value of
81.52% was detected in Riceberry and the highest ABTS value of 67.92% in KDML 105 RPSL-
2. Additionally, a strong correlation between antioxidant, antityrosinase and germination
time was detected. The information obtained from this study should provide a foundation for
future research aiming at the development of functional foods and/or cosmetics.
2018-01-01T00:00:00Zประโยชน์ของจุลินทรีย์โพรไบโอติกต่อสุขภาพแก่นแสนดี, วิรัชนีย์มูลมั่งมี, สมพรแสงศิลา, อรุณรัศมีอิศรานุวัฒน์, ปริยาภรณ์http://dspace.bru.ac.th/xmlui/handle/123456789/41152018-04-03T07:45:28Z2558-03-02T00:00:00Zประโยชน์ของจุลินทรีย์โพรไบโอติกต่อสุขภาพ
แก่นแสนดี, วิรัชนีย์; มูลมั่งมี, สมพร; แสงศิลา, อรุณรัศมี; อิศรานุวัฒน์, ปริยาภรณ์
Probiotic products are generally defined as food supplements containing beneficial microbes that give heath benefits to consumers. A good probiotic should be non-pathogenic, non-toxic and capable of exerting beneficial effect on the host. It should be present as viable cells and capable of surviving and metabolizing in the gut environment. The minimum count of probiotics in products is recommended at 106 cfu/g. The beneficial effect of probiotics consumption includies improving intestinal tract health, production of antimicrobial agents such as hydrogen peroxide and/or bacteriocin, protecting host from intestinal infection, stimulate immune responses and reducing symptoms of lactose intolerance. This article presents an overview on the health benefits of probiotics and their impact on consumers.; ผลิตภัณฑ์โพรไบโอติก หมายถึง ผลิตภัณฑ์เสริมอาหารที่ประกอบด้วยจุลินทรีย์ที่มีประโยชน์และส่งผลต่อสุขภาพของผู้บริโภคโพรไบโอติกที่ดีไม่ควรก่อให้เกิดโรค ไม่ก่อให้เกิดสารพิษ และสามารถให้ผลที่เป็นประโยชน์ต่อเจ้าบ้าน ซึ่งเป็นจุลินทรีย์ที่มีชีวิตและสามารถรอดชีวิตและผลิตสารที่เป็นประโยชน์ในระบบลำไส้ ปริมาณโพรไบโอติกตํ่าสุดในผลิตภัณฑ์ที่แนะนำ เท่ากับ 106 cfu/g ประโยชน์ของการบริโภคโพรไบโอติก ได้แก่ การผลิตสารต้านจุลินทรีย์ เช่น ไฮโดรเจนเปอร์ออกไซด์ และ/หรือแบคเทอริโอซิน ป้องกันการติดเชื้อในลำไส้ กระตุ้นการตอบสนองของระบบภูมิคุ้มกัน และลดอาการแพ้แลคโตส ซึ่งบทความนี้แสดงภาพรวมเกี่ยวกับประโยชน์ของโพรไบโอติกต่อสุขภาพของผู้บริโภค
2558-03-02T00:00:00Z-Sangsila, ArunrussameeFaucet-Marquis, VirginiePfohl-Leszkowicz, AnnieItsaranuwat, Pariyapornhttp://dspace.bru.ac.th/xmlui/handle/123456789/41132018-04-03T06:50:00Z2015-10-24T00:00:00Z-
Sangsila, Arunrussamee; Faucet-Marquis, Virginie; Pfohl-Leszkowicz, Annie; Itsaranuwat, Pariyaporn
Zearalenone (ZEA) contamination in food samples plays a critical role in food safety, since it causes serious health problems. Usage of microorganisms, such as lactic acid bacteria (LAB), is a promising new approach for detoxification. Eight Lactobacillus pentosus strains were evaluated for their ability to remove ZEA from a sodium acetate buffer solution with initial ZEA concentrations of 5.51–74.70 μg/mL. The adsorption capacity increased with increasing ZEA concentrations. The strain JM0812 showed the highest adsorption capability, at 83.17%, in solution containing 74.70 μg/mL ZEA, followed by UM054 (82.78%) and UM055 (81.69%), respectively. Three adsorption isotherms were applied to predict the removal efficiency of ZEA and the Freundlich isotherm appeared to have the best fit for ZEA sorption onto bacterial cells. Our results indicate that Lb. pentosus strains are novel promising strains to reduce mycotoxin contamination in food products.
2015-10-24T00:00:00ZHalotolerant Cyanobacterium Aphanothece halophytica Contains an Na+-dependent F1F0-ATP Synthase with a Potential Role in Salt-stress ToleranceSoontharapirakkul, KanteeraPromden, WorrawatYamada, NanaKageyama, HakutoIncharoensakdi, AranIwamoto-Kihara, AtsukoTakabe, Teruhirohttp://dspace.bru.ac.th/xmlui/handle/123456789/41042018-03-28T08:00:51Z2011-01-01T00:00:00ZHalotolerant Cyanobacterium Aphanothece halophytica Contains an Na+-dependent F1F0-ATP Synthase with a Potential Role in Salt-stress Tolerance
Soontharapirakkul, Kanteera; Promden, Worrawat; Yamada, Nana; Kageyama, Hakuto; Incharoensakdi, Aran; Iwamoto-Kihara, Atsuko; Takabe, Teruhiro
Aphanothecehalophyticaisahalotolerantalkaliphiliccyanobacteriumthatcangrowinmediaofupto3.0MNaClandpH11. Here,weshowthatinadditiontoatypicalH -ATPsynthase, Aphanothece halophytica contains a putative F1F0-type Na ATP synthase (ApNa+-ATPase) operon (ApNa+-atp). The operonconsistsofninegenesorganizedintheorderofputative subunits , ,I,hypotheticalprotein,a,c,b, ,and .Homologousoperonscouldalsobefoundinsomecyanobacteriasuch as Synechococcus sp. PCC 7002 and Acaryochloris marina MBIC11017. The ApNa -atp operon was isolated from the A.halophyticagenomeandtransferredintoanEscherichiacoli mutant DK8 ( atp) deficient in ATP synthase. The inverted membrane vesicles of E.coli DK8 expressing ApNa -ATPase exhibited Na -dependent ATP hydrolysis activity, which was inhibitedbymonensinandtributyltinchloride,butnotbythe protonophore,carbonylcyanidem-chlorophenylhydrazone (CCCP). The Na ion protected the inhibition of ApNa ATPasebyN,N -dicyclohexylcarbodiimide.TheATPsynthesis activitywasalsoobservedusingtheNa -loadedinvertedmembranevesicles.ExpressionoftheApNa -atpoperonintheheterologouscyanobacteriumSynechococcussp.PCC7942showed its localization in the cytoplasmic membrane fractions and increased tolerance to salt stress. These results indicate that A.halophytica has additional Na -dependent F1F0-ATPase in thecytoplasmicmembraneplayingapotentialroleinsalt-stress tolerance.
2011-01-01T00:00:00ZAn Mrp-Like Cluster in the Halotolerant Cyanobacterium Aphanothece halophytica Functions as a Na+/H+ AntiporterFukaya, FuminoriPromden, WorrawatHibino, TakashiTanaka, YoshitoNakamura, TatsunosukeTakabe, Teruhirohttp://dspace.bru.ac.th/xmlui/handle/123456789/41032018-03-28T07:54:35Z2009-10-01T00:00:00ZAn Mrp-Like Cluster in the Halotolerant Cyanobacterium Aphanothece halophytica Functions as a Na+/H+ Antiporter
Fukaya, Fuminori; Promden, Worrawat; Hibino, Takashi; Tanaka, Yoshito; Nakamura, Tatsunosuke; Takabe, Teruhiro
The mrp homolog gene cluster mrpCD1D2EFGAB (Ap-mrp) was found in a halotolerant cyanobacterium, Aphanothece halophytica, amplified, and expressed in Escherichia coli mutant TO114. Ap-mrp complemented the salt-sensitive phenotype of TO114 and exhibited Na+/H+ and Li+/H+ exchange activities, indicating that Ap-Mrp functions as a Na /H antiporter.
2009-10-01T00:00:00ZIsolation and characterization of proline/betaine transporter gene from oil palmYamada, NanaCha-Um, SuriyanKageyama, HakutoPromden, WorrawatTanaka, YoshitoKirdmanee, ChalermpolTakabe, Teruhirohttp://dspace.bru.ac.th/xmlui/handle/123456789/41022018-03-28T07:49:41Z2011-04-01T00:00:00ZIsolation and characterization of proline/betaine transporter gene from oil palm
Yamada, Nana; Cha-Um, Suriyan; Kageyama, Hakuto; Promden, Worrawat; Tanaka, Yoshito; Kirdmanee, Chalermpol; Takabe, Teruhiro
Oil production from oil palm is adversely affected by drought and salt. Under drought and salt stress, proline content increases in oil palm; the mechanism for this is unknown. Here, an 8319-nucleotide sequence including cDNA, genomic DNA and the promoter region of proline transporter gene from oil palm Elaeis guineensis was determined. The transporter gene exhibited high similarity to Bet/ProT genes from several plants, but the highest homology was found with rice ProT1. The exon–intron structure of genomic DNA was unique, and numerous stress-response cis-elements were found in the promoter region. Expression of cDNA EgProT1 in Escherichia coli mutant exhibited uptake activities for glycinebetaine and choline as well as proline. Under salt-stressed conditions, exogenously applied glycinebetaine was taken up into the root more rapidly than the control. These data indicate that oil palm has a unique Pro/T1 gene. Nucleotide sequence data for the cDNA and genomic DNA of proline transporter gene from Elaeis guineensis are available in the DDJB database under accession numbers AB597035 and AB597036, respectively.
2011-04-01T00:00:00ZPreferential accumulation of betaine uncoupled to choline monooxygenase in young leaves of sugar beet--importance of long-distance translocation of betaine under normal and salt-stressed conditionsYamada, NanaPromden, WorrawatYamane, KojiTamagake, HidetoHibino, TakashiTanaka, YoshitoTakabe, Teruhirohttp://dspace.bru.ac.th/xmlui/handle/123456789/41012018-03-28T07:42:57Z2009-06-01T00:00:00ZPreferential accumulation of betaine uncoupled to choline monooxygenase in young leaves of sugar beet--importance of long-distance translocation of betaine under normal and salt-stressed conditions
Yamada, Nana; Promden, Worrawat; Yamane, Koji; Tamagake, Hideto; Hibino, Takashi; Tanaka, Yoshito; Takabe, Teruhiro
It has been reported that glycinebetaine (betaine) is synthesized in response to abiotic stresses via a two-step oxidation of choline in which choline monooxygenase (CMO) and betaine aldehyde dehydrogenase (BADH) are involved. Here we show that significant amounts of betaine, > 20 micromol/gFW, accumulated in young leaves of Beta vulgaris even under normal growth conditions, whereas levels in old leaves, cotyledons, hypocotyls, and roots were low. Under the same conditions, CMO accumulates exclusively in old leaves and is difficult to be detected in young leaves. By contrast, the levels of BADH were high in all tissues. Exogenously supplied choline was converted into betaine in old leaves, but levels were significantly lower in young leaves under the same conditions. When d(11)-betaine was applied exogenously to old leaves, it was translocated preferentially into young leaves and roots. In response to salt stress, betaine levels increased in all tissues, but most significantly increased in young leaves. The levels of CMO increased in various tissues, but were low in young leaves. A betaine transporter gene was isolated. Its expression was more strongly induced in old leaves than in young leaves. Based on these data, we discussed the role of CMO and betaine transporter under stress and non-stress conditions.
2009-06-01T00:00:00ZMolecular Characterization and Heterologous Expression of Quinate Dehydrogenase Gene from Gluconobacter oxydans IFO3244Vangnai, AlisaPromden, WorrawatDe-Eknamkul, WanchaiMatsushita, KazunobuToyama, Hirohidehttp://dspace.bru.ac.th/xmlui/handle/123456789/41002018-03-28T07:33:50Z2009-06-01T00:00:00ZMolecular Characterization and Heterologous Expression of Quinate Dehydrogenase Gene from Gluconobacter oxydans IFO3244
Vangnai, Alisa; Promden, Worrawat; De-Eknamkul, Wanchai; Matsushita, Kazunobu; Toyama, Hirohide
The quinate dehydrogenase (QDH) from Gluconobacter oxydans IFO3244 exhibits high affinity for quinate, sug gesting its application in shikimate production. Nucleotide sequence analysis of the qdh gene revealed a full length of 2475 bp encoding an 824 amino acid protein. The qdh gene has the unusual TTG translation initiation codon. Conserved regions and a signature sequence for the quinoprotein family were observed. Phylogenetic analysis demonstrated relatedness of QDH from G. oxydans to other quinate/shikimate dehydrogenases with the highest similarity (56%) with that of Acinetobacter calcoaceticus ADP1 and lower similarity (36%) with a membrane bound glucose dehydrogenase of Escherichia coli. The function of the gene coding for QDH was confirmed by heterologous gene expression in pyrroloquinoline quinone synthesizing Pseudomonas putida HK5.
2009-06-01T00:00:00ZDisruption of quinoprotein ethanol dehydrogenasegene and adjacent genes in Pseudomonas putida HK5Promden, WorrawatVangnai, AlisaPongsawasdi, PiamsookAdachi, OsaoMatsushita, KazunobuToyama, Hirohidehttp://dspace.bru.ac.th/xmlui/handle/123456789/40992018-03-28T07:30:45Z2008-01-01T00:00:00ZDisruption of quinoprotein ethanol dehydrogenasegene and adjacent genes in Pseudomonas putida HK5
Promden, Worrawat; Vangnai, Alisa; Pongsawasdi, Piamsook; Adachi, Osao; Matsushita, Kazunobu; Toyama, Hirohide
Pseudomonas putida HK5 produces three different quinoprotein alcohol dehydrogenases: ADH-I, ADH-IIB and ADH-IIG. Gene organization of qedA, the gene for ADH-I, and other 10 genes in the cluster was related to the genome sequences of five other Pseudomonas strains. Insertion mutations in either qedA, exaE or agmR eliminated ADH-I activity, although the mutants were still able to grow on ethanol but more slowly than the wild-type strain. Mutant analysis demonstrated the requirement of agmR and exaE in ADH-I expression, and the tentative involvement of agmR, but not exaE, in the induction of ADH-IIB and ADH-IIG activities.
2008-01-01T00:00:00ZAnalysis of the promoter activities of the genes encoding three quinoprotein alcohol dehydrogenases in Pseudomonas putida HK5Promden, WorrawatVangnai, AlisaToyama, HirohideMatsushita, KazunobuPongsawasdi, Piamsookhttp://dspace.bru.ac.th/xmlui/handle/123456789/40982018-03-28T07:24:15Z2008-10-01T00:00:00ZAnalysis of the promoter activities of the genes encoding three quinoprotein alcohol dehydrogenases in Pseudomonas putida HK5
Promden, Worrawat; Vangnai, Alisa; Toyama, Hirohide; Matsushita, Kazunobu; Pongsawasdi, Piamsook
The transcriptional regulation of three distinct alcohol oxidation systems, alcohol dehydrogenase (ADH)-I, ADH-IIB and ADH-IIG, in Pseudomonas putida HK5 was investigated under various induction conditions. The promoter activities of the genes involved in alcohol oxidation were determined using a transcriptional lacZ fusion promoter-probe vector. Ethanol was the best inducer for the divergent promoters of qedA and qedC, encoding ADH-I and a cytochrome c, respectively. Primary and secondary C3 and C4 alcohols and butyraldehyde specifically induced the divergent promoters of qbdBA and aldA, encoding ADH-IIB and an NAD-dependent aldehyde dehydrogenase, respectively. The qgdA promoter of ADH-IIG responded well to (S)-(+)-1,2propanediol induction. In addition, the roles of genes encoding the response regulators exaE and agmR, located downstream of qedA, were inferred from the properties of exaE- oragmRdisrupted mutants and gene complementation tests. The gene products of both exaE and agmR were strictly necessary for qedA transcription. The mutation and complementation studies also suggested a role for AgmR, but not ExaE, in the transcriptional regulation of qbdBA (ADH-IIB) and qgdA (AGH-IIG). A hypothetical scheme describing a regulatory network, which directs expression of the three distinct alcohol oxidation systems in P. putida HK5, was derived.
2008-10-01T00:00:00ZStructure and Antioxidant Activity Relationships of Isoflavonoids from Dalbergia parvifloraPromden, WorrawatMonthakantirat, OrawanUmehara, KaoruNoguchi, HiroshiDe-Eknamkul, Wanchaihttp://dspace.bru.ac.th/xmlui/handle/123456789/40972018-03-28T07:14:32Z2014-02-01T00:00:00ZStructure and Antioxidant Activity Relationships of Isoflavonoids from Dalbergia parviflora
Promden, Worrawat; Monthakantirat, Orawan; Umehara, Kaoru; Noguchi, Hiroshi; De-Eknamkul, Wanchai
The antioxidant activities of 24 isoflavonoids that were previously isolated as pure compounds from Dalbergia parviflora were evaluated using three different in vitro antioxidant-based assay systems: xanthine/xanthine oxidase (X/XO), ORAC, and DPPH. The isolates consisted of three subgroups, namely isoflavones, isoflavanones, and isoflavans, each of which appeared to have diversified substituents, and were thus ideal for the study of their structure-activity relationships (SARs). The SAR analysis was performed using the results obtained from both the inter-subgroup isoflavonoids with the same substitution pattern and the intra-subgroup compounds with different substitution patterns. The inter-subgroup comparison showed that the isoflavones exhibited the highest antioxidant activities based on all three assays. The intra-subgroup analysis showed that the additional presence of an OH group in Ring B at either R3′ or R5′ from the basic common structure of the R7-OH of Ring A and the R4′-OH (or -OMe) of Ring B greatly increased the antioxidant activities of all of the isoflavonoid subgroups and that other positions of OH and OMe substitutions exerted different effects on the activities depending on the subgroup and assay type. Therefore, based on the structural diversity of the isoflavonoids in D. parviflora, the present study provides the first clarification of the detailed antioxidant SARs of isoflavonoids.
2014-02-01T00:00:00ZFunctional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus OhbaSintupachee, SirilukPromden, WorrawatNgamrojanavanich, NattayaSitthithaworn, WorapanDe-Eknamkul, Wanchaihttp://dspace.bru.ac.th/xmlui/handle/123456789/40962018-03-28T07:07:37Z2015-10-01T00:00:00ZFunctional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus Ohba
Sintupachee, Siriluk; Promden, Worrawat; Ngamrojanavanich, Nattaya; Sitthithaworn, Worapan; De-Eknamkul, Wanchai
While attempting to isolate the enzyme geranylgeraniol 18-hydroxylase, which is involved in plaunotol biosynthesis in Croton stellatopilosus (Cs), the cDNAs for a cytochrome P450 monooxygenase(designated as CYP76F45) and an NADPH-cytochrome P450 reductase (designated as CPR I based on its classification) were isolated from the leaf. The CYP76F45 and CsCPR I genes have open reading frames (ORFs) encoding 507- and 711-amino acid proteins with predicted relative molecular weights of 56.7 and 79.0 kDa,respectively. Amino acid sequence comparison showed that both CYP76F45 (63–73%) and CsCPR I (79–83%) share relatively high sequence identities with homologous proteins in other plant species.Phylogenetic tree analysis confirmed that CYP76F45 belongs to the CYP76 family and that CsCPR I belongs to Class I of dicotyledonous CPRs, with both being closely related to Ricinus communis genes. Functional characterization of both enzymes, each expressed separately in Escherichia coli as recombinant proteins,showed that only simultaneous incubation of the membrane bound proteins with the substrate geraniol (GOH) and the coenzyme NADPH could form 8-hydroxygeraniol. The enzyme mixture could also utilize acyclic sesquiterpene farnesol (FOH) with a comparable substrate preference ratio (GOH:FOH) of 54:46. The levelsof the CYP76F45 and CsCPR I transcripts in the shoots, leaves and twigs of C. stellatopilosus were correlated with the levels of a major monoterpenoid indole alkaloid, identified tentatively as 19-Evallesamine,that accumulated in these plant parts. These results suggested that CYP76F45 and CPR I function as the enzyme geraniol-8-hydroxylase (G8H), which is likely to be involved in the biosynthesis of the indole alkaloid in C. stellatopilosus
2015-10-01T00:00:00ZExpression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patientCHANVORACHOTE, PITHILUANPITPONG, SUDJITCHUNHACHA, PREEDAKORNPROMDEN, WORRAWATSRIURANPONG, VIROTEhttp://dspace.bru.ac.th/xmlui/handle/123456789/40602018-03-28T04:32:52Z2012-05-01T00:00:00ZExpression of CA125 and cisplatin susceptibility of pleural effusion-derived human lung cancer cells from a Thai patient
CHANVORACHOTE, PITHI; LUANPITPONG, SUDJIT; CHUNHACHA, PREEDAKORN; PROMDEN, WORRAWAT; SRIURANPONG, VIROTE
Advances in understanding lung cancer biology and tumor markers aid clinicians in managing the disease. Cancer-associated antigen (CA)125 has garnered increasing attention in lung cancer research and may benefit the treatment and follow-up of this type of cancer. In Thai lung cancer patients, knowledge regarding ethnic differences in cancer cell biology is largely absent. We generated lung cancer cells from the pleural effusion fluids of a Thai patient and designated these as P1 cells. P1 cells were assessed for growth rate, response to chemotherapy, and the presence of tumor markers, in particular CA125 expression. Results of immunofluorescence indicated that P1 cells exhibited strong expression levels of CA125, comparable to that of established H460 lung cancer cells. Furthermore, P1 cells were analyzed for the expression of additional markers. Results revealed that H460 cells exhibited strong immunofluorescent signals from cytokeratin-19 fragments (CYFRA 21-1) and squamous cell carcinoma antigen (SCCA) while P1 presented only CYFRA 21-1 signals. We also found evidence of relative cisplatin resistance in P1 compared to the susceptibility level of established lung cancer cells. Thus, the results and methodology described in this study may aid the development of lung cancer diagnostic and therapeutic approaches and, in particular, advance understanding of ethnic differences.
2012-05-01T00:00:00Zชีวเคมีของเอนไซม์แอลกอฮอล์ดีไฮโดรจีเนสในแบคทีเรียวรวัฒน์, พรหมเด่นhttp://dspace.bru.ac.th/xmlui/handle/123456789/40572018-03-28T04:20:21Z2013-11-01T00:00:00Zชีวเคมีของเอนไซม์แอลกอฮอล์ดีไฮโดรจีเนสในแบคทีเรีย
วรวัฒน์, พรหมเด่น
เอนไซม์แอลกอฮอล์ดีไฮโดรจีเนสทำหน้าที่เร่งปฏิกิริยาออกซิเดชันหรือรีดักชันทำให้เกิดการเปลี่ยนแปลงระหว่างแอลกอฮอล์และแอลดีไฮด์หรือคีโตน สามารถจำแนกเอนไซม์กลุ่มนี้ตามชนิดของตัวรับอิเล็กตรอนได้เป็น 3 กลุ่ม คือ แอลกอฮอล์ดีไฮโดรจีเนสชนิดที่ขึ้นกับ NAD(P)+ แอลกอฮอล์ดีไฮโดรจีเนสชนิดที่ไม่ขึ้นกับ NAD(P)+ และแอลกอฮอล์ออกซิเดสชนิดที่ขึ้นกับ FAD มีการศึกษาพบว่าเอนไซม์แอลกอฮอล์ดีไฮโดรจีเนสในแบคทีเรียมีหลากหลายชนิดซึ่งมีความแตกต่างกันหลายประการ เช่น ลำดับนิวคลีโอไทด์ ลำดับกรดอะมิโน โครงสร้างสามมิติของโปรตีน คุณสมบัติทางจลนศาสตร์การเร่งปฏิกิริยาของเอนไซม์ ตำแหน่งที่พบเอนไซม์ในเซลล์ ตลอดจนกลไกการควบคุมการแสดงออกระดับยีน ในบทความนี้กล่าวถึงองค์ความรู้ทางชีวเคมีพื้นฐานที่เกี่ยวข้องกับเอนไซม์แอลกอฮอล์ดีไฮโดรจีเนสในแบคทีเรีย โดยเฉพาะอย่างยิ่งในด้านการออกซิเดชันของแอลกอฮอล์; Alcohol dehydrogenases (ADHs) catalyze the oxidative and reductive conversion of alcohols and aldehydes or ketones. They can be divided into three major categories; The NAD(P)+- dependent alcoholdehydrogenases, NAD(P)+ - independent alcohol dehydrogenases and FAD-dependent alcohol oxidases. There are several varieties of alcohol dehydrogenases found in bacteria each of which may exist in several variants,
e.g., molecular properties, catalytic properties, localization as well as gene expression and regulation. This article describes the basic knowledge of biochemistry that is related to bacterial alcohol dehydrogenases, especially in
oxidation of alcohols.
2013-11-01T00:00:00Z